5 Simple Statements About attune flow cytometer Explained
5 Simple Statements About attune flow cytometer Explained
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At the moment, the early symptoms ended up dismissed as debris, though the retrospective analysis demonstrated shared characteristics with the problematic cells in society. Tracing the an infection assisted the lab establish more laboratory techniques for screening and safety of assay-essential cell traces.
No matter whether you’re a rookie or an authority, check out our sources to advance your exploration and experience in flow cytometry.
(B) RAW 264.seven macrophages are sometimes annotated inaccurately with regard to particle count because of their complexity and noncircular borders. The volume of singlets readily available for Evaluation boosts when the person trains and employs a more exact product.
The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing which has a substantial-velocity brightfield camera for simultaneous significant throughput flow cytometry and higher resolution brightfield imaging.
"A picture isn't pretty value 1000 dots, nevertheless it seriously helps to see photographs. It is excellent to see illustrations or photos that correlate with staining. For example, AnnexinV-positive cells which have been morphologically distinct than wholesome cells, or CD14+ monocytes that happen to be more substantial and much more textured than CD3+/CD4+/CD14- lymphocytes.
Furthermore, it is illuminating to view the amount of doublets slip by way of classic singlet gates, and fantastic to confirm the standing of unexpected double-constructive occasions in my analysis."
Pair your Attune flow cytometer with the autosampler for greater workflow performance, and include robotic integration for optimum throughput.
Steady image high-quality even at significant throughput Acoustic concentrating plus a high-speed digital camera Mix to graphic these T cells constantly at lower or higher flow costs. Easily alter focus and camera options to meet experimental needs.
Early signs of this contamination had at first been dismissed as debris. Backgating to find new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also lets you use morphological characteristics to find exciting subpopulations that will not be evident from flow cytometry facts by itself.
Customers can perspective event pictures in authentic-time to confirm sample quality, improve protocols, and report the morphology of each cell inhabitants identified for Investigation. Imaging capture also Added benefits from acoustic focusing, which helps to place the cells so that a pointy, centered graphic is acquired.
Audio waves empower precise mobile alignment, resulting in Extraordinary information high-quality and ~10X more quickly sample flow rates.
Application Observe: Automatic image Evaluation lowers person-to-user variability in flow cytometry gating methods
Designed-in dimensionality reduction equipment enhanced resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to verify expected population resolution in lysed entire human blood. Person can view occasions get more info from Every single cluster for visual affirmation of mobile phenotypes.
Versatile payment system from Agilent Monetary Alternatives permit you to receive the crucial instrumentation you may need for rapid job, or to expand your lab.
At the heart of each and every Attune Flow Cytometer lies our groundbreaking acoustic concentrating technological innovation, which makes use of audio waves to meticulously align cells inside the flow cell. This innovative method surpasses regular hydrodynamic strategies, causing a lot quicker processing speeds, Outstanding info high-quality, and elevated sample throughput.
The Attune Xenith cytometer is actually a superior-parameter spectral analyzer, delivering both spectral unmixing and conventional flow capabilities for thorough and functional mobile Assessment.
Agilent Worth Assure ensures you at the least a decade of instrument use in the day you buy. Or else, We are going to credit you While using the residual value of the method toward an upgraded design.
What appeared to be a homogeneous populace is in fact much more various—an insight that ought to be considered when interpreting results.